Blood-brain barrier amenable gold nanoparticles biofabrication in aged cell culture medium

Cell-culture models of the blood-brain barrier.

A cell culture model of the blood-brain barrier

The actions of an intracellular nitric oxide generator compound on the properties of a co-culture model of the blood-brain barrier are described. Addition of the iron-sulphur cluster nitrosyl Roussin's black salt (RBS, heptanitrosyl-tri-mu3-thioxotetraferrate (1-)) resulted in a rapid and dose-dependent (50-250 microM) decline in the electrical resistance displayed by co-cultures of vascular en...

A Cell Culture Model of the Blood-Brain Barrier

Endothelial cells that make up brain capillaries and constitute the blood-brain barrier become different from peripheral endothelial cells in response to inductive factors found in the nervous system . We have established a cell culture model of the bloodbrain barrier by treating brain endothelial cells with a combination of astrocyte-conditioned medium and agents that elevate intracellular cAM...

Biofabrication of gold and silver nanoparticles for pharmaceutical applications

Biofabrication by using fungi is an exciting recent interest to develop an eco-friendly production of metallic nanoparticles (NPs) for pharmaceutical applications. This study aimed to synthesize and characterize gold (Au) and silver (Ag) NPs by using Penicillium simplisimum. The fungus was grown in fluid czapek dox broth on shaker at 28 ºC and 200 rpm for ten days. Then the supernatant was...

Spontaneous penetration of gold nanoparticles through the blood brain barrier (BBB)

BACKGROUND The blood brain barrier (BBB) controls the brain microenvironment and limits penetration of the central nervous system (CNS) by chemicals, thus creating an obstacle to many medical imaging and treatment procedures. Research efforts to identify viable routes of BBB penetration have focused on structures such as micelles, polymeric nanoparticles and liposomes as drug carriers, however,...